YOU Jing-mao;XIONG Kun;MU Sen;GUO Jie;GUO Xiao-liang;DUAN Yuan-yuan;LI Juan;CAO Fan;ZOU Zong-cheng;TAN Hai;

• 1:Institute of Chinese Herbal Medicines
• 2:Hubei Academy of Agricultural Sciences
• 3:Enshi for Food and Drug Inspection and Testing Center
• 4:Ba Dong Jin Da Pharmaceutical Co.

Abstract：

In this study,an endophytic bacteria strain BZJN1 was isolated from Atractylodes macrocephala,and identified as Bacillus subtilis by physiological and biochemical tests and molecular identification. Strain BZJN1 could inhibit the growth of mycelia of Ceratobasidium sp. significantly,and the inhibition rate was more than 70%. The mycelium growth deformity with bulge as spherical and partially exhaustible in apex or central with microscopic observation. The inhibitory rates under 3% and 6% concentrations of the cell free fermentation were 22. 7% and 38. 7% expectively. The field test proved that the control efficacy of treatment of 1 × 10~9cfu·mL~(-1) is 75. 27% and 72. 37% after 10 and 20 days. All the treatments of strain BZJN1 was able to promote the growth of A. macrocephala,the treatment of 1 × 10~9cfu·mL~(-1) could able to increase the yield to 14. 1%.

Key Words： Bacillus subtilis;Atractylodes macrocephala;Ceratobasidium sp;control

Abstract:

Keywords:

Foundation: 湖北省自然科学基金面上项目(2016CFB521);; 湖北省农业科学院竞争性项目(2016JZXJH019);湖北省农业科学院青年科学基金项目(2018NKYJJ16);; 国家现代农业产业技术体系建设专项(CARS-21);; 湖北省技术创新专项(民族专项MZZX2018000005)

Authors: YOU Jing-mao;XIONG Kun;MU Sen;GUO Jie;GUO Xiao-liang;DUAN Yuan-yuan;LI Juan;CAO Fan;ZOU Zong-cheng;TAN Hai;

DOI: 10.19540/j.cnki.cjcmm.20180105.008

References：

• [1]中国药典.一部[S].2010:96.
• [2]You J M,Lin X M,Guo J.First report of root rot on Atractylodes macrocephala(Largehead Atractylodes Rhizome)caused by Ceratobasidium sp.in China[J].Plant Dis,2013,97(1):139.
• [3]Wu H S,Zhou X D,Shi X,et al.In vitro responses of Fusarium oxysporum f.sp.niveum to phenolic acids indecaying watermelon tissues[J].Phytochem Lett,2014,8(13):172.
• [4]檀国印.白术真菌病害的分离鉴定及根际促生菌的筛选[D].北京:中国林业科学研究院,2013.
• [5]倪方方,徐红梅,腾蛟.生防菌对白术根腐病菌的拮抗作用及盆栽防治效果[J].浙江中医药大学学报,2017(3):179.
• [6]东秀珠,蔡妙英.常见细菌系统鉴定手册[M].北京:科学出版社,2001:62.
• [7]刘洋,朱天辉,郑磊.一株内生拮抗细菌的分离鉴定及其抗菌机理研究[J].植物保护,2016,42(1):33.
• [8]Chun J,Bae K S.Phylogenetic analysis of Bacillus subtilis and related taxa based on partial gyr A gene sequence[J].Antonie van Leeuwenhoek,2000,78:123.
• [9]游景茂,向国耀,郭杰.8种杀菌剂对白术根腐病防治研究[J].植物保护,2016,42(6):235.
• [10]张一名,李小娟,王颖.抑制向日葵核盘菌菌核形成的生防菌S-16的鉴定及其生防作用研究[J].中国生物防治学报,2014,30(1):121.
• [11]张成省,孔凡玉,刘朝科.枯草芽孢杆菌Tpb55挥发物对烟草的防病促生效应[J].中国生物防治学报,2009,25(3):245.
• [12]鲁红学,杨廷宪,杨佩.内生枯草芽孢杆菌QZ-8对茄子的抗病促生作用[J].湖北农业科学,2013,52(21):5199.