WANG Kang-yu;LIU Wei-can;ZHANG Mei-ping;ZHAO Ming-zhu;WANG Yan-fang;LI Li;SUN Chun-yu;HU Ke-xin;CONG Yue-yi;WANG Yi;

• 1:College of Life Science
• 2:Jilin Agricultural University
• 3:Research Center for Ginseng Genetic Resources Development and Utilization
• 4:College of Traditional Chinese Medicine

Abstract：

The relationship between saponin content of Panax quinquefolius in different parts of the organization and expression of ginsenoside biosynthesis related gene was obtained by the correlation analysis between saponin content and gene expression.The 14 tissue parts of P.quinquefolius were studied,six saponins in P.quinquefolius.Samples(ginsenoside Rg1,Re,Rb1,Rc,Rb2 and Rd),group saponins and total saponins were determined by high performance liquid chromatography and vanillin-sulfuric acid colorimetric method.Simultaneously,the expression levels of 7 ginsenoside biosynthesis related genes(SQS,OSC,DS,β-AS,SQE,P450 and FPS) in different tissues of P.quinquefolius were determined by Real-time fluorescence quantitative PCR.Although 7 kinds of ginsenoside biosynthesis related enzyme gene in the P.quinquefolius involved in ginsenoside synthesis,the expression of β-AS and P450 genes had no significant effect on the content of monosodium saponins,grouping saponins and total saponins,FPS,SQS,OSC,DS and SQE had significant or extremely significant on the contents of single saponins Re,Rg1,Rb1,Rd,group saponin PPD and PPT,total saponin TMS and total saponin TS(P<0.05 or P<0.01).The biosynthesis of partial saponins,grouping saponins and total saponins in P.quinquefolius was affected by the interaction of multiple enzyme genes in the saponin synthesis pathway,the content of saponins in different tissues of P.quinquefolius was determined by the differences in the expression of key enzymes in the biosynthetic pathway.Therefore,this study further clarified that FPS,SQS,OSC,DS and SQE was the key enzyme to control the synthesis of saponins in P.quinquefolius by correlation analysis,the biosynthesis of ginsenosides in P.quinquefolius was regulated by these five kind of enzymes in cluster co-expression of interaction mode.

Key Words： Panax quinquefolius;gene expression;ginsenoside biosynthesis pathway;correlation;real-time quantitative PCR

Abstract:

Keywords:

Foundation: 国家高技术研究发展计划(863)项目(2013AA102604-3);; 吉林省发改委-吉林省省级产业创新专项(2016C04);; 吉林省科技厅自然科学基金主题引导项目(20170101010JC);; 吉林农业大学博士科研启动基金项目(201618)

Authors: WANG Kang-yu;LIU Wei-can;ZHANG Mei-ping;ZHAO Ming-zhu;WANG Yan-fang;LI Li;SUN Chun-yu;HU Ke-xin;CONG Yue-yi;WANG Yi;

DOI: 10.19540/j.cnki.cjcmm.20171030.006

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