JIANG Chun-li;SU Xin-yao;XU Ya-chun;WU Er-yi;SHI Yu-hua;ZHANG Dong;CHEN Qing-fu;SUN Wei;XUE Jian-ping;

• 1:College of Life Sciences
• 2:Huaibei Normal University
• 3:Key Laboratory of Beijing for Identification and Safe Evaluation of Chinese Medicine
• 4:Institute of Chinese Meteria Medica

Abstract：

Tartary buckwheat Fagopyrum tataricum is an important medicinal and functional herb due to its rich content of flavonoids in the seeds. F. tataricum exhibited good functions for free radicals scavenging,anti-oxidation,anti-aging activities. Although much genetic knowledge of the synthesis,regulation,accumulation of rutin,the genetic basis of proanthocyanidins( PAs) in tartary buckwheat and their related gene expression changes under different lights( blue,red,far red,ultraviolet light) remain largely unexplored. In this study,we cloned one anthocyanidin reductase gene( ANR) and two leucocyanidin reductase gene( LAR) named Ft ANR,FtLAR1,FtLAR3 involved in formation of( +)-catechin and(-)-epicatechin precusor proanthocyanidin by digging out F. tataricum seed transcriptome data. The expression data showed that the opposite influence of red light on these gene transcript level compared to others lights. The expression levels of Ft ANR and FtLAR1 decreased and FtLAR3 appeared increment after exposed in the red light,while the expression levels of those genes appeared opposite result after exposed in the blue and far red light.

Key Words： Fagopyrum tataricum;proanthocyanidin;light induction;gene expression;quantitative analysis

Abstract:

Keywords:

Foundation: 国家自然科学基金项目(81573518,81603248);; 安徽省自然科学基金项目(1408085MC58);; 中央级公益性科研院所基本科研业务费专项(GH2017-01-01,GH2017-01)

Authors: JIANG Chun-li;SU Xin-yao;XU Ya-chun;WU Er-yi;SHI Yu-hua;ZHANG Dong;CHEN Qing-fu;SUN Wei;XUE Jian-ping;

DOI: 10.19540/j.cnki.cjcmm.20180105.005

References：

• [1]Fabjan N,Rode J,Ko2ir I J,et al.Tartary buckwheat(Fagopyrum tataricum Gaertn.)as a source of dietary rutin and quercitrin[J].J Agr Food Chem,2003,51(22):6452.
• [2]Bonafaccia G,Marocchini M,Kreft I.Composition and technological properties of the flour and bran from common and tartary buckwheat[J].Food Chem,2003,80(1):9.
• [3]Jing R,Li H Q,Hu C L,et al.Phytochemical and pharmacological profiles of three Fagopyrum buckwheats[J].Int J Mol Sci,2016,17(4):589.
• [4]Dixon R A,Achnine L,Kota P,et al.The phenylpropanoid pathway and plant defence—a genomics perspective[J].Mol Plant Pathol,2002,3(5):371.
• [5]Taylor L P,Grotewold E.Flavonoids as developmental regulators[J].Curr Opin Plant Biol,2005,8(3):317.
• [6]Kreft S,trukelj B,Gaber2cˇik A,et al.Rutin in buckwheat herbs grown at different UV-B radiation levels:comparison of two UV spectrophotometric and an HPLC method[J].J Exp Bot,2002,53(375):1801.
• [7]Gaber2cˇik A,Voncˇina M,Tro2t T,et al.Growth and production of buckwheat(Fagopyrum esculentum)treated with reduced,ambient,and enhanced UV-B radiation[J].J Photoch Photobio B,2002,66(1):30.
• [8]Watanabe M.Catechins as antioxidants from buckwheat(Fagopyrum esculentum Moench)groats[J].J Agr Food Chem,1998,46(3):839.
• [9]Burda S,Oleszek W.Antioxidant and antiradical activities of flavonoids[J].J Agr Food Chem,2001,49(6):2774.
• [10]Arima H,Ashida H,Danno G.Rutin-enhanced antibacterial activities of flavonoids against Bacillus cereus and Salmonella enteritidis[J].Biosci Biotech Bioch,2002,66(5):1009.
• [11]雒晓鹏,卜星星,赵海霞,等.LED光源对芽期苦荞黄酮合成的影响[J].食品科学,2015(3):86.
• [12]Luk2icˇL,rvay J,VollmannováA,et al.Hydrothermal treatment of tartary buckwheat grain hinders the transformation of rutin to quercetin[J].J Cereal Sci,2016,72:131.
• [13]孙朝霞,侯思宇,令狐斌,等.苦荞全生育期芦丁积累与其生物合成途径相关基因表达分析[J].中国农业科学,2017,50(18):3473.
• [14]Murray J R,Hackett W P.Dihydroflavonol reductase activity in relation to differential anthocyanin accumulation in juvenile and mature phase Hedera helix L[J].Plant physiol,1991,97(1):343.
• [15]Lister C E,Lancaster J E,Walker J R L.Phenylalanine ammonia-lyase(PAL)activity and its relationship to anthocyanin and flavonoid levels in New Zealand-grown apple cultivars[J].J Am Soc Hortic Sci,1996,121(2):281.
• [16]刘金,魏景立,刘美艳,等.早熟苹果花青苷积累与其相关酶活性及乙烯生成之间的关系[J].园艺学报,2012,39(7):1235.
• [17]Wei H,Chen X,Zong X,et al.Comparative transcriptome analysis of genes involved in anthocyanin biosynthesis in the red and yellow fruits of sweet cherry(Prunus avium L.)[J].PLo S ONE,2015,10(3):e0121164.
• [18]Lacampagne S,GagnéS,Gény L.Involvement of abscisic acid in controlling the proanthocyanidin biosynthesis pathway in grape skin:new elements regarding the regulation of tannin composition and leucoanthocyanidin reductase(LAR)and anthocyanidin reductase(ANR)activities and expression[J].J Plant Growth Regul,2010,29(1):81.
• [19]Tian L,Pang Y,Dixon R A.Biosynthesis and genetic engineering of proanthocyanidins and(iso)flavonoids[J].Phytochem Rev,2008,7(3):445.
• [20]Bagchi D,Bagchi M,Stohs S J,et al.Free radicals and grape seed proanthocyanidin extract:importance in human health and disease prevention[J].Toxicology,2000,148(2):187.
• [21]Tanner G J,Francki K T,Abrahams S,et al.Proanthocyanidin biosynthesis in plants purification of legume leucoanthocyanidin reductase and molecular cloning of its c DNA[J].J Biol Chem,2003,278(34):31647.
• [22]Pang Y,Peel G J,Wright E,et al.Early steps in proanthocyanidin biosynthesis in the model legume Medicago truncatula[J].Plant Physiol,2007,145(3):601.
• [23]Bogs J,Downey M O,Harvey J S,et al.Proanthocyanidin synthesis and expression of genes encoding leucoanthocyanidin reductase and anthocyanidin reductase in developing grape berries and grapevine leaves[J].Plant Physiol,2005,139(2):652.
• [24]Xie D Y,Sharma S B,Paiva N L,et al.Role of anthocyanidin reductase,encoded by Banyuls in plant flavonoid biosynthesis[J].Science,2003,299(5605):396.
• [25]Paolocci F,Robbins M P,Madeo L,et al.Ectopic expression of a basic helix-loop-helix gene transactivates parallel pathways of proanthocyanidin biosynthesis.Structure,expression analysis,and genetic control of leucoanthocyanidin 4-reductase and anthocyanidin reductase genes in Lotus corniculatus[J].Plant Physiol,2007,143(1):504.
• [26]Rosati C,Cadic A,Duron M,et al.Molecular characterization of the anthocyanidin synthase gene in Forsythia×intermedia reveals organ-specific expression during flower development[J].Plant Sci,1999,149(1):73.
• [27]温鹏飞,邢延富,牛铁泉,等.UV-C对葡萄果实发育过程中黄烷醇类多酚积累及隐色花色素还原酶表达的影响[J].中国农业科学,2012,45(21):4428.
• [28]Zhang Z Z,Li X X,Chu Y N,et al.Three types of ultraviolet irradiation differentially promote expression of shikimate pathway genes and production of anthocyanins in grape berries[J].Plant Physiol Bioch,2012,57:74.
• [29]Eguchi K,Sato T.Differences in the ratios of cyanidin-3-O-glucoside and cyanidin-3-O-rutinocide to total anthocyanin under UV and non-UV conditions in tartary buckwheat(Fagopyrum tataricum Garten)[J].Plant Prod Sci,2009,12(2):150.
• [30]Saitou N,Nei M.The neighbor-joining method:a new method for reconstructing phylogenetic trees[J].Mol Biol Evol,1987,4(4):406.
• [31]Felsenstein J.Confidence limits on phylogenies:an approach using the bootstrap[J].Evolution,1985,39(4):783.
• [32]Kumar S,Stecher G,Tamura K.MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets[J].Mol Biol Evol,2016,33(7):1870.
• [33]Matsui K,Hisano T,Yasui Y,et al.Isolation and characterization of genes encoding leucoanthocyanidin reductase(Fe LAR)and anthocyanidin reductase(Fe ANR)in buckwheat(Fagopyrum esculentum)[J].J Plant Physiol,2016,205:41.
• [34]Tegelberg R,Julkunen-Tiitto R,Aphalo P J.The effects of longterm elevated UV-B on the growth and phenolics of field-grown silver birch(Betula pendula)[J].Global Change Biol,2001,7(7):839.
• [35]Jansen M A K,Hectors K,O'Brien N M,et al.Plant stress and human health.Do human consumers benefit from UV-B acclimated crops[J].Plant Sci,2008,175(4):449.
• [36]Pontin M A,Piccoli P N,Francisco R,et al.Transcriptome changes in grapevine(Vitis vinifera L.)cv.Malbec leaves induced by ultraviolet-B radiation[J].BMC Plant Biol,2010,10(1):224.
• [37]Koyama K,Ikeda H,Poudel P R,et al.Light quality affects flavonoid biosynthesis in young berries of Cabernet sauvignon grape[J].Phytochemistry,2012,78:54.
• [38]Wang W,Tang K,Yang H R,et al.Distribution of resveratrol and stilbene synthase in young grape plants(Vitis vinifera L.cv.Cabernet sauvignon)and the effect of UV-C on its accumulation[J].Plant Physiol Bioch,2010,48(2):142.
• [39]Pan Q H,Wang L,Li J M.Amounts and subcellular localization of stilbene synthase in response of grape berries to UV irradiation[J].Plant Sci,2009,176(3):360.
• [40]Ubi B E,Honda C,Bessho H,et al.Expression analysis of anthocyanin biosynthetic genes in apple skin:effect of UV-B and temperature[J].Plant Sci,2006,170(3):571.
• [41]Higashio H,Hirokane H,Sato F,et al.Effect of UV irradiation after the harvest on the content of flavonoid in vegetables[J].Acta Hortic,2005,682(682):1007.
• [42]Kataoka I,Beppu K.UV Irradiance Increases Development of red skin color and anthocyanins in Hakuho'Peach[J].Hort Science,2004,39(6):1234.
• [43]Sun M,Gu X,Fu H,et al.Change of secondary metabolites in leaves of Ginkgo biloba L.in response to UV-B induction[J].Innov Food Sci Emerg,2010,11(4):672.
• [44]陈静,陈启林,翁俊,等.不同红光/远红光比例(R/FR)的光照影响番茄幼苗叶片中花青素合成的研究[J].西北植物学报,2004,24(10):1773.