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| Allicin Enhances Cytotoxicity of CPT-11 to Colon Cancer LoVo Cell in vitro |
| Received:June 11, 2009 Revised:June 11, 2009 |
| DOI： |
| KeyWord:Allicin Colon carcinoma LoVo cell Apoptosis Cell cycle CPT-11 |
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| Abstract: |
| Objective： To study the effect of allicin on human colon cancer cell line LoVo and that combination of allicin with CPT-11 enhances cytotoxicity of CPT-11 to LoVo cell in vitro. Methods：The LoVo cell were cultured in vitro and treated by allicin in different concentrations. MTT assay was used to test dynamically the effect of the cell growth inhibiting. Apoptosis induction (Annexin-V-FITC/PI) and modulation of DNA cell cycle was measured by flow cytometry. The change of cytotoxicity of CPT-11 after combination of allicin 4.0μg/ml and 8.0μg/ml were investigated. Results：Allicin had inhibitive effects on growth of LoVo cells in a dose and time dependent manner，with the IC50 value of 32.23, 10.74 and 6.58μg/ml at 24h, 48h and 72h respectively. The apoptosis rate of LoVo cells increased progressively treated with increasing concentration allicin for 24 h, while the apoptosis rate achieved peak value when treated with 8μg/ml allicin for 48 h. The cell cycle was arrested at G2/M phase treated with allicin at concentration lower than 4μg/ml and at G1 G2/M phase treated with allicin at concentration higher than 4μg/ml for 24 h. In comparison with IC50 of CPT-11 alone or combined with allicin 4.0μg/ml and 8.0μg/ml, the 24h IC50 values decreased from 47.5 ug/ml to 7.4 and 7.2 ug/ml and the inhibition efficiency increased 6.4 and 6.6 times respectively. Conclusions：Allicin has a significant anti-proliferation effect on human colon cancer cell line LoVo by induction of apoptosis and arrestment of cell cycle and may enhance cytotoxicity of CPT-11 on the Colon Cancer LoVo Cell. |
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